Trans-anethole, the major constituent of the essential oil of anise, improves the antiarthritic effects of methotrexate in rats

The aim of this study was to evaluate the effect trans-anethole in combination with methotrexate, compared monotherapy or on inflammatory parameters and oxidative stress arthritic rats. experimental model arthritis induced by Freund's complete adjuvant (AIA) rats used. Treatment monotherapy, started day AIA induction continued for 21 days. association anethole (62.5 mg/Kg) methotrexate (6 therapy able further reduce both events that characterize disease, as demonstrated following indicators: 1) decrease edema hind legs, score secondary lesions, recruitment leukocytes articular cavity plasma myeloperoxidase activity; 2) increase content reduced thiols total antioxidant capacity; 3) diminution protein carbonylation plasma, liver kidneys; 4) lipid peroxidation liver; 5) reduction hepatic ROS content; 6) enhancement activity enzymes GSH/GSSG ratio. In general, + presented a substantially higher than (at same doses). data obtained work allow us conclude use suppressed progression rats, main advantage dose, attenuating adverse effects caused high dose therapy.